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タイトル: Immunohistochemical localization of mesenchymal stem cells in ossified human spinal ligaments
著者: Chin, Shunfu
Furukawa, Ken-Ichi
Ono, Atsushi
Asari, Toru
Harada, Yoshifumi
Wada, Kanichiro
Tanaka, Toshihiro
Inaba, Wataru
Mizukami, Hiroki
Motomura, Shigeru
Yagihashi, Soroku
Ishibashi, Yasuyuki
著者所属: 弘前大学大学院医学研究科
Department of Pharmacology, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki, Aomori 036-8562, Japan
Department of Orthopaedic Surgery, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki, Aomori 036-8562, Japan
Department of Pathology and Molecular Medicine, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki, Aomori 036-8562, Japan
発行日: Jun-2013
出版者: Elsevier
誌名: Biochemical and biophysical research communications
巻: 436
号: 4
開始ページ: 698
終了ページ: 704
ISSN: 0006-291X
DOI: 10.1016/j.bbrc.2013.06.019
キーワード: Spinal ligament ossification
Mesenchymal stem cells
Immunohistochemistry
Localization
Neovascularization
Chondrocytes
抄録: Mesenchymal stem cells (MSCs) have been isolated from various tissues and used for elucidating the pathogenesis of numerous diseases. In our previous in vitro study, we showed the existence of MSCs in human spinal ligaments and hypothesized that these MSCs contributed to the pathogenesis of ossification of spinal ligaments. The purpose of this study was to use immunohistochemical techniques to analyze the localization of MSCs in ossified human spinal ligaments in situ. Ossified (OLF) or non-ossified ligamentum flavum (non-OLF) samples from the thoracic vertebra were obtained from patients who had undergone posterior spinal surgery. Serial sections were prepared from paraffin-embedded samples, and double immunofluorescence staining was performed using antibodies against markers for MSCs (CD73, CD90 and CD105), endothelial cells (CD31), pericytes (α-smooth muscle actin), and chondrocytes (S100). Immunolocalization of MSCs was observed in the perivascular area and collagenous matrix in spinal ligaments. Markers for MSCs and pericytes were co-expressed in the perivascular area. Compared with non-OLF, OLF had a large amount of neovascularization in the fragmented ligament matrix, and a high accumulation of MSCs around blood vessels. The prevalence of MSCs in OLF within collagenous matrix was significantly higher than that in non-OLF. Chondrocytes near the ossification front in OLF also presented expression of MSC markers. MSCs may contribute to the ectopic ossification process of OLF through endochondral ossification.
URI: http://hdl.handle.net/10129/5113
テキストバージョン: ETD
学位授与番号: 11101A1811
学位授与年月日: 2013-09-30
学位名: 博士(医学)
学位授与機関: 弘前大学
出現コレクション:平成25年度前期

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