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30 医学部・医学研究科・保健学研究科 >
30b 弘前医学 = Hirosaki Medical Journal >
59巻Supplement >
Please use this identifier to cite or link to this item:
http://hdl.handle.net/10129/2240
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| Title: | Protective eff ect of intranasal vaccination with nontoxic mutant TSST-1 against Staphylococcus aureus infection |
| Authors: | Narita, Kouji
Hu, Dong-Liang
Tsuji, Takao
Nakane, Akio |
| Authors's Post: | Department of Microbiology and Immunology, Hirosaki University Graduate School of Medicine
Department of Microbiology and Immunology, Hirosaki University Graduate School of Medicine
Department of Microbiology, Fujita Health University, School of Medicine, Toyoake
Department of Microbiology and Immunology, Hirosaki University Graduate School of Medicine |
| Issue Date: | 29-Nov-2007 |
| Publisher: | 弘前大学大学院医学研究科・弘前医学会 |
| Shimei: | 弘前医学 |
| Volume: | 59 |
| Issue: | Supplement |
| Start page: | S227 |
| End page: | S234 |
| ISSN: | 0439-1721 |
| Abstract: | Infection caused by methicillin-resistant Staphylococcus aureus （MRSA） has been the most commonly
acquired types of nosocomial infections. It was reported that anterior nares are the major reservoir of S. aureus and
the source of 80% of S. aureus bacteremia is endogenous. Considering these facts, elimination and reduction of nasal
carriage are thought to be eff ective protection against systemic S. aureus infection and nosocomial infection. Toxic shock
syndrome toxin 1（ TSST-1） is one of superantigens secreted by S. aureus. Previously, it was reported that mutant form
（H135A） of TSST-1（ mTSST-1） was shown to be nontoxic, and subcutaneous vaccination with mTSST-1 could protect
against systemic S. aureus infection in a mouse model. In this study, we investigated the protective eff ect of intranasal
vaccination with mTSST-1 supplemented with non-toxic mutant（ H44A） Escherichia coli heat labile toxin（ mLT） as a
mucosal adjuvant. The results demonstrated that intranasal immunization with mTSST-1 plus mLT could efficiently
induce production of anti-TSST-1 antibodies in sera and also induce anti-TSST-1 IgA production in bronchoalveolar lavage
fl uids（ BALF） of vaccinated mice. In nasal-associated lymphoid tissues（ NALT） of vaccinated mice, anti-TSST-1 IgA
secreting cells were signifi cantly increased. To evaluate of the protective eff ect of this vaccine against systemic S. aureus
infection, BALB/c mice were vaccinated with mTSST-1 plus mLT and challenged with clinical isolated S. aureus 834
intravenously. Bacterial numbers in spleen and liver, and cumulative mortality rate of vaccinated mice were lower than
those of control mice. We further developed a mouse model of nasal S. aureus colonization. S. aureus bacterial numbers in
nasal cavity of vaccinated mice were signifi cantly reduced compared with those of control mice. These results indicate
that intranasal immunization with mTSST-1 plus mLT is able to induce systemic and mucous immune responses and of
provide protection against systemic S. aureus infection and nasal colonization. |
| URI: | http://hdl.handle.net/10129/2240 |
| textversion: | publisher |
| Appears in Collections: | 59巻Supplement
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