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弘大学術情報リポジトリは OAI-PMHに準拠しています

Hirosaki University Repository for Academic Resources >
30 医学部・医学研究科・保健学研究科 >
30b 弘前医学 = Hirosaki Medical Journal >
59巻Supplement >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10129/2240

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HirosakiMedJ_59_S227.pdf		269Kb	Adobe PDF	View/Open

Title:	Protective eff ect of intranasal vaccination with nontoxic mutant TSST-1 against Staphylococcus aureus infection
Authors:	Narita, Kouji Hu, Dong-Liang Tsuji, Takao Nakane, Akio
Authors's Post:	Department of Microbiology and Immunology, Hirosaki University Graduate School of Medicine Department of Microbiology and Immunology, Hirosaki University Graduate School of Medicine Department of Microbiology, Fujita Health University, School of Medicine, Toyoake Department of Microbiology and Immunology, Hirosaki University Graduate School of Medicine
Journal title:	弘前医学
volume:	59
issue:	Supplement
start page:	S227
end page:	S234
ISSN:	0439-1721
Issue Date:	29-Nov-2007
Publisher:	弘前大学大学院医学研究科・弘前医学会
Abstract:	Infection caused by methicillin-resistant Staphylococcus aureus （MRSA） has been the most commonly acquired types of nosocomial infections. It was reported that anterior nares are the major reservoir of S. aureus and the source of 80% of S. aureus bacteremia is endogenous. Considering these facts, elimination and reduction of nasal carriage are thought to be eff ective protection against systemic S. aureus infection and nosocomial infection. Toxic shock syndrome toxin 1（ TSST-1） is one of superantigens secreted by S. aureus. Previously, it was reported that mutant form （H135A） of TSST-1（ mTSST-1） was shown to be nontoxic, and subcutaneous vaccination with mTSST-1 could protect against systemic S. aureus infection in a mouse model. In this study, we investigated the protective eff ect of intranasal vaccination with mTSST-1 supplemented with non-toxic mutant（ H44A） Escherichia coli heat labile toxin（ mLT） as a mucosal adjuvant. The results demonstrated that intranasal immunization with mTSST-1 plus mLT could efficiently induce production of anti-TSST-1 antibodies in sera and also induce anti-TSST-1 IgA production in bronchoalveolar lavage fl uids（ BALF） of vaccinated mice. In nasal-associated lymphoid tissues（ NALT） of vaccinated mice, anti-TSST-1 IgA secreting cells were signifi cantly increased. To evaluate of the protective eff ect of this vaccine against systemic S. aureus infection, BALB/c mice were vaccinated with mTSST-1 plus mLT and challenged with clinical isolated S. aureus 834 intravenously. Bacterial numbers in spleen and liver, and cumulative mortality rate of vaccinated mice were lower than those of control mice. We further developed a mouse model of nasal S. aureus colonization. S. aureus bacterial numbers in nasal cavity of vaccinated mice were signifi cantly reduced compared with those of control mice. These results indicate that intranasal immunization with mTSST-1 plus mLT is able to induce systemic and mucous immune responses and of provide protection against systemic S. aureus infection and nasal colonization.
URI:	http://hdl.handle.net/10129/2240
textversion:	publisher
Appears in Collections:	59巻Supplement